ABSTRACT
<p><b>OBJECTIVE</b>To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.</p><p><b>METHODS</b>Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.</p><p><b>RESULTS</b>In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.</p><p><b>CONCLUSION</b>There is a correlation between La protein and HBV mRNA and the expression of HBV protein.</p>
Subject(s)
Humans , Autoantigens , Metabolism , Cell Line, Tumor , DNA, Viral , Hepatitis B Surface Antigens , Hepatitis B virus , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , RNA, Small Interfering , RNA, Viral , Ribonucleoproteins , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To identify and analyse the different species, same species in different regions and confusion species.</p><p><b>METHOD</b>Near-infrared diffuse reflectance spectrometry was used.</p><p><b>RESULT</b>Clustering analysis showed that clustering relations were far among different Gryllotalpa species and close among the same species from different regions, and there were close relations among the same species from near regions and between Teleogryllus emmus and G. orientalis.</p><p><b>CONCLUSION</b>Near-infrared diffuse reflectance spectrometry method can be used in classification and identification of Gryllotalpa.</p>
Subject(s)
Animals , Cluster Analysis , Drug Contamination , Gryllidae , Chemistry , Classification , Materia Medica , Chemistry , Classification , Pharmacognosy , Species Specificity , Spectroscopy, Near-InfraredABSTRACT
<p><b>AIM</b>To study the importance of blood pressure variability in organ protection for long-term treatment with fosinopril in-sinoaortic-denervated (SAD) rats.</p><p><b>METHODS</b>Fosinopril (15 mg.kg-1.d-1) was given in rat chow for 16 weeks after SAD surgery. Blood pressure variability (BPV) was recorded during 24 h in conscious state. Histopathological changes were evaluated with light microscope and computer-assisted image analysis.</p><p><b>RESULTS</b>Long-term treatment with fosinopril significantly decreased BPV in SAD rats. The thickness of the left ventricular wall, collagen fraction of the left ventricle and glomerulosclerosis score were all positively related to BPV in untreated and fosinopril-treated SAD rats. Fosinopril markedly prevented the damages of target organs in SAD rats.</p><p><b>CONCLUSION</b>Long-term treatment with fosinopril showed obvious organ protection in SAD rats. The decrease in BPV may significantly contribute to organ protection.</p>